p57 cKO, B6-Cdkn1c floxed
p57 cKO, B6-Cdkn1c floxed
Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J (N6 generations).
B (1-3 months)
RBRC06402
中山 敬一
Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
p57 floxed mice. Exons 2 to 4 were flanked by loxP sites (Flox type). Paternal imprinting gene.
B（1〜3か月）
Cre/loxP system
<a href='https://brc.riken.jp/mus/pcr06402'>Genotyping protocol -PCR-</a>
Cancer Research
Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.bioreg.kyushu-u.ac.jp/saibou/index_en.html" target="_blank">Lab HP</A>
E14 [129P2/OlaHsd]
九州大学生体防御医学研究所・中山敬一先生。E14 ES細胞を用いて作出。C57BL/6Jへ交配された（N6世代）。
p57 floxed mice. Exons 4 to 4 were flanked by loxP sites (Flox type). Paternal imprinting gene.
true
B6;129P2-Cdkn1c<tm2.1Kei>
B6;129P2-Cdkn1c<tm2.1Kei>
Metabolism Research
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>共同研究。
phage P1 loxP sites, mouse Cdkn1c genomic DNA
Cell Biology Research
Keiichi NAKAYAMA
p57 floxedマウス。エクソン2から4がloxPで挟まれている (Flox型) 。ホモノックアウトマウスの多くは胎生期致死。細胞が増殖を行うためには、サイクリン/CDK複合体と呼ばれる分子群が活性化することが必須である。CDKインヒビターと呼ばれるp57は、サイクリン/CDK複合体の機能を阻害することによって、細胞の増殖を抑制している。p57は父性インプリンティング遺伝子。